World Journal of Biology and Biological Sciences

World Journal of Biology and Biological Sciences Vol. 4 (1), pp. 09-14, October 2016, ISSN 2331-1894 ©2015 World Science Research Journals 

 

Full Length Research 

 

Influence of bioactive metabolites extracted from three species of nematode-trapping fungi on the growth of Escherichia coli and Staphylococcus aureus 

 

Ali A. Kasim

 

Biological Department, College of Sciences, Misan University, Iraq. 

 

E-mail: alimycol@yahoo.com.  

 

Accepted September 25, 2016 

 

Abstract  

 

The effect of different concentrations of cultures filtrate, crude filtrate extracts and fungal mycelia crude extracted from three species of nematode-trapping fungi (Arthrobotrys dactyloides, Arthrobotrys oligospora and Dactylella brochopaga) on the growth of Escherichia coli and Staphylococcus aureus by using a disc diffusion technique was examined. All fungal extracts exhibited an inhibitory action against both bacterial isolates. It was noted that the crude filtrate extract is more efficient in inhibitory action than cultures filtrate and fungal mycelia crude extract. Moreover, cultures filtrate and crude filtrate extracts possessed higher inhibitory effect against E. coli when compared with S. aureus. It was also noted that the crude filtrate extracts inhibited the growth of bacteria more efficiently than that of cultures filtrate. Extracts of A. oligospora showed the best inhibition effect, followed by D. brochopaga then A. dactyloides. The cultures filtrate of A. oligosporashowed the highest inhibitory ability against E. coli and S. aureus which amounted to 13.17 and 11 mm, respectively, followed by D. brochopaga  which amounted to 9.67 and 8.58 mm, respectively. The crude filtrate extracts of A. oligospora exhibited an inhibitory action reaching 16.42 and 14.50 mm, respectively, followed by D. brochopaga (13.17 and 11.42 mm, respectively). A comparison between the inhibitory action of three commercial drugs and the fungal extracts showed that the cultures filtrate and crude filtrate extracts rendered a greater bioactivity than the examined drugs.

 

Key words: Nematode-trapping fungi, Arthrobotrys oligospora, antibacterial, antibiotics, bioactive metabolites, growth inhibition zones.